expression levels  (Cusabio)

Journal: Scientific Reports

Article Title: AMPK affects the development of early-stage NAFLD by activating autophagy and fatty acid oxidation

doi: 10.1038/s41598-025-31181-0

Figure Lengend Snippet: Moderate lipid accumulation promoted both lipid oxidation and synthesis. AML12 ( A – F ) and HepG2 ( G – K ) cells were stimulated with either 100 µM or 200 µM palmitic acid (PA) alone, or with combinations of 100 µM PA and 200 µM oleic acid (OA), or 200 µM PA and 400 µM OA for 24 h. ( A , G ) Cell viability was measured with CCK-8 assay. ( B , H ) Cellular triglyceride. (TG) levels were quantified. ( C ) Oil Red O staining marked lipid droplets in cells (scale bar, 200 μm), and lipid area analysis was performed. ( D , E , I , K ) Western blot analyses for PPARα, ACC, and SREBP-1c in cells, along with statistical band intensity analysis, divided into 5 groups, NT, 100 µM PA, 200 µM PA, 100 µM PA + 200 µM OA, 200 µM PA + 400 µM OA. ( F , J ) mRNA expression levels of proteins involved in lipid synthesis and oxidation were measured; n = 3 biologically independent experiments. * P < 0.05; ** P < 0.01, compared to NT (normal treatment). Mice were intraperitoneally injected with Tyloxapol (Ty) at concentrations of 100 mg/kg, 200 mg/kg, and 300 mg/kg for 12 h. ( L ) Diagram of Ty intraperitoneal injection procedure in mice. ( M ) Hepatic mRNA expression levels of proteins involved in lipid synthesis and oxidation. ( N , O ) Western blot results for PPARα, ACC, and SREBP-1c in mice liver, along with statistical band intensity analysis, divided into 4 groups, NT, 100 mg/kg Ty, 200 mg/kg Ty, 300 mg/kg Ty. n = 10; * P < 0.05; ** P < 0.01, versus NT. Data were expressed as mean ± standard deviation (SD).

Article Snippet: The cDNA was used as a template in real-time quantitative PCR to assess mRNA expression levels (TaKaRa, Cat# RR420A).

Techniques: CCK-8 Assay, Staining, Western Blot, Expressing, Injection, Standard Deviation

Journal: Scientific Reports

Article Title: AMPK affects the development of early-stage NAFLD by activating autophagy and fatty acid oxidation

doi: 10.1038/s41598-025-31181-0

Figure Lengend Snippet: Autophagy levels were influenced by the degree of lipid accumulation. AML12 ( A – C ) and HepG2 ( D – F ) cells were stimulated with either 100 µM or 200 µM PA alone, or with combinations of 100 µM PA and 200 µM OA, or 200 µM PA and 400 µM OA for 24 h. ( A , D ) AML12 and HepG2 LC3 , P62 mRNA expression levels. ( B , C , E , F ) Bands detection and grayscale analysis of AML12 and HepG2 autophagy protein, divided into 5 groups, NT, 100 µM PA, 200 µM PA, 100 µM PA + 200 µM OA, 200 µM PA + 400 µM OA; n = 3 biologically independent experiments. * P < 0.05; ** P < 0.01, versus NT. Mice were intraperitoneally injected with Ty at concentrations of 100 mg/kg, 200 mg/kg, and 300 mg/kg for 12 h. ( G ) Mice liver Lc3 , p62 mRNA expression levels. ( H , I ) Bands detection and grayscale analysis of mice liver autophagy protein, divided into 4 groups, NT, 100 mg/kg Ty, 200 mg/kg Ty, 300 mg/kg Ty; n = 10; * P < 0.05; ** P < 0.01, versus NT. Data were expressed as mean ± SD.

Article Snippet: The cDNA was used as a template in real-time quantitative PCR to assess mRNA expression levels (TaKaRa, Cat# RR420A).

Techniques: Expressing, Injection

Journal: Scientific Reports

Article Title: AMPK affects the development of early-stage NAFLD by activating autophagy and fatty acid oxidation

doi: 10.1038/s41598-025-31181-0

Figure Lengend Snippet: Effects of different degrees of lipid accumulation on energy metabolism. AML12 ( A – D ) and HepG2 ( E – H ) cells were stimulated with either 100 µM or 200 µM PA alone, or with combinations of 100 µM PA and 200 µM OA, or 200 µM PA and 400 µM OA for 24 h. ( A , E ) ATP levels in AML12 cells and HepG2 cells. ( B , F ) Cellular CO1 , CO2 , CO3 , CO4 mRNA expression levels. ( C , D , G , H ) Bands detection and grayscale analysis of cellular energy metabolism proteins, divided into 5 groups, NT, 100 µM PA, 200 µM PA, 100 µM PA + 200 µM OA, 200 µM PA + 400 µM OA; n = 3 biologically independent experiments. * P < 0.05; ** P < 0.01, versus NT. Mice were intraperitoneally injected with Ty at concentrations of 100 mg/kg, 200 mg/kg, and 300 mg/kg for 12 h. ( I ) ATP levels in mice liver. ( J ) Mice liver Co1 , Co2 , Co3 , Co4 mRNA expression levels. ( K , L ) Bands detection and grayscale analysis of mice liver energy metabolism proteins, divided into 4 groups, NT, 100 mg/kg Ty, 200 mg/kg Ty, 300 mg/kg Ty; n = 10; * P < 0.05; ** P < 0.01, versus NT. AML12 cells were stimulated with 200 µM PA and 400 µM OA, as well as 330 µM PA and 660 µM OA for 24 h, respectively. ( M ) ATP levels in AML12 cells; n = 3 biologically independent experiments. * P < 0.05; ** P < 0.01, versus NT. ## P < 0.01, versus PA 200 µM + OA 400 µM. Mice were fed HFD for 4 weeks and 12 weeks, respectively. ( N ) ATP levels in mice liver; n = 7; ** P < 0.01, versus NC. ## P < 0.01, versus 4 weeks HFD. Data were expressed as mean ± SD.

Article Snippet: The cDNA was used as a template in real-time quantitative PCR to assess mRNA expression levels (TaKaRa, Cat# RR420A).

Techniques: Expressing, Injection